Quantification of cytochrome 2E1 in human liver microsomes using a validated indirect ELISA
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چکیده
منابع مشابه
Involvement of cytochrome P450 2E1 in the ( v –1)-hydroxylation of oleic acid in human and rat liver microsomes
In vitro techniques have been used to investigate the nature of microsomal cytochrome P450 involved in the metabolism of oleic acid, a physiological monounsaturated fatty acid. Like lauric acid, which is currently used as a model substrate of fatty acid metabolism, the alkyl chain of oleic acid is hydroxylated on its v and ( v –1) carbons. The identity of these hydroxylated metabolites was asce...
متن کاملInvolvement of cytochrome P450 2E1 in the (omega-1)-hydroxylation of oleic acid in human and rat liver microsomes.
In vitro techniques have been used to investigate the nature of microsomal cytochrome P450 involved in the metabolism of oleic acid, a physiological monounsaturated fatty acid. Like lauric acid, which is currently used as a model substrate of fatty acid metabolism, the alkyl chain of oleic acid is hydroxylated on its omega and (omega-1) carbons. The identity of these hydroxylated metabolites wa...
متن کاملEffect of Cytochrome b5 Content on the Activity of Polymorphic CYP1A2, 2B6, and 2E1 in Human Liver Microsomes
Human cytochrome b5 (Cyt b5) plays important roles in cytochrome P450 (CYP)-mediated drug metabolism. However, the expression level of Cyt b5 in normal human liver remains largely unknown. The effect of Cyt b5 on overall CYP activity in human liver microsomes (HLM) has rarely been reported and the relationship between Cyt b5 and the activity of polymorphic CYP has not been systematically invest...
متن کاملA new cytochrome in liver microsomes.
8. HEPPEL, L. A., in S. P. COLOWICK AND N. 0. KAPLAN (Editors), Methods in enzymology, Vol. II, Academic Press, Inc., New York, 1955, p. 575. CANTONI, G. L., in M. FLORKIN AND H. S. MASON (Editors), Comparative biochemistry, Vol. I, Academic Press, Inc., New York, 1960, p. 181. THEORELL, H., in D. E. GREEN, (Editor), Currents in biochemical Research, Interscience Publishers, Inc., New York, 195...
متن کاملInhibition of Cytochrome P450 by Propolis in Human Liver Microsomes
Although propolis is one of the most popular functional foods for human health, there have been no comprehensive studies of herb-drug interactions through cytochrome P450 (CYP) inhibition. The purpose of this study was to determine the inhibitory effects of propolis on the activities of CYP1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 and 3A4 using pooled human liver microsomes (HLMs). Propolis inhibited ...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2014
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2013.09.008